Retention and Selectivity for Hydrophilic Interaction Liquid Chromatography (HILIC) Columns: Multivariate Modeling and Correlation with Physiochemical Properties

摘要

A HILIC column classification model was presented for guided selection of stationary phase chemistry based on retention and selectivity. The model confirmed the strong impact of electrostatic interactions in HILIC, but also revealed that adsorption and partitioning are very important for selectivity. These properties varied significantly between stationary phases, sometimes even if intended to be similar, and could be correlated to physiochemical properties of the columns. Poly -functionalized zwitterionic stationary phases like ZICHILIC showed selectivity depending on partitioning and weak ion exchange and were least affected by changes in organic solvent environment. All non -functionalized bare silica columns had similar selectivity which depended on cation exchange, plus adsorption rather than partitioning.