cDNA Cloning and Expression Analysis of Three Components of SCF~(COI1) Complex in Hevea brasiliensis

摘要

To gain a deeper understanding of jasmonate signaling in specific tissues, three of full-length cDNAs, referred to as HbCULl, HbSKP1 and HbRBXl were isolated from laticifers of rubber tree by the rapid amplification of cDNA ends (RACE) method and analysed by sequence analysis and real-time RT-PCR. The putative proteins, HbCULl, HbSKPl and HbRBXl, had specific domains of CULLIN superfamily and Cullin Nedd8, Skpl-POZ and Skpl, and RING superfamily, respectively. HbCULl and HbSKPl were most related to AtCULl and AtASKl among the members of Cullins and ASKs from Arabidopsis, respectively. Methyl jasmonate was more effective than ethylene on up-regulating the expression of HbCULl, HbSKPl and HbRBXl.