HPLC-UV detection for analysis of p-benzoquinone dioxime and p-nitrosophenol, and chromatographic fingerprint applied in quality control of industrial p-benzoquinone dioxime

摘要

A reliable reversed-phase high performance liquid chromatographic (HPLC) method has been developed for simultaneous determination of p-benzoquinone dioxime (BQD) and its related impurity p-nitrosophenol (NSP). Separation was achieved on a Kromasil C-18 column by using methanol-water-NH4Ac-NH3 solution (pH = 7.0, 50 mM) (30/50/20, v/v/v) as the mobile phase, and detection was operated by UV absorption at a wavelength of 305 nm. The method was seen to have good linearity, accuracy, and precision for the concentration range and to be an attractive choice for the quality control of BQD for industrial use. Moreover, the HPLC-UV-vis fingerprint of BQD has been established, and successfully applied to quality control of industrial BQD in laboratories of some rubber factories in China. Chromatographic fingerprints of intermediates would become an effective strategy for accelerating the progress of fine chemical industry.