Separation and determination of trace Cu(II), Zn(II), and Pd(II)as their tetraphenylporphine chelates by reversed-phase high performance liquid chromatography

摘要

#alpha#,#beta#.#delta#-Tetraphenylporphine(TPP)was used as a precolumn chelating reagnet for reversed-phase high performance liquid chromatographic (HPLC) separation and determination of trace Cu~(2+), Zn~(2+) and Pd~(2+). Chelating reactions of TPP with Cu~(2+), Zn~(2+), and Pd~(2+) have been optimized in t he presence of anionic surfactant sodium dodecyl sulphate(SDS). TPP and its metal chelates were successfully separated on a Nucleosil C_(18) column (150 X 4.6 mm I.D., 5 #mu#m)with acetone-acetonitrile(37.5:62.5,v/v)as mobile phase.