The effect of liraglutide on the proliferation, migration, and osteogenic differentiation of human periodontal ligament cells

摘要

Abstract Objective Liraglutide ( LIRA ) is a novel antidiabetic therapy that may have anti‐inflammatory and bone protective effects. Thus, we studied the potential therapeutic effect of LIRA on periodontitis by assessing the effects of LIRA on the proliferation, migration, inflammation, and osteogenic differentiation of human periodontal ligament cells ( hPDLC s) after LPS stimulation. Material and Methods The expression of glucagon like‐peptide 1 receptor ( GLP ‐1R) was measured using qRT ‐ PCR . HPDLC s proliferation after LIRA were analyzed using MTT assays. Cell migration was quantified using a wound‐healing assay. The expression of inflammatory ( IL ‐6 and TNF ‐α) was measured by qRT ‐ PCR and ELISA in hPDLC s. The effect of LIRA on the mineralization potential of hPDLC s was assessed by alizarin red S staining. Furthermore, the expression of Runx2 and ALP was measured by qRT ‐ PCR and Western blot in hPDLC s. Results GLP ‐1R mRNA was present on hPDLC s, and LIRA increased the expression of GLP ‐1R mRNA . When cultured with 25, 50, 75, 100 and 125 nM LIRA for 24 h, hPDLC s proliferation was enhanced in a dose‐dependent manner ( P?? 0.05), and 100 nM was optimal. LIRA promoted hPDLC s migration in a time‐dependent manner. LPS significantly increased the expression of IL ‐6 and TNF ‐α ( P?? 0.01), decreased the formation of mineralization nodes ( P?? 0.01), and inhibited the expression of ALP and Runx2 ( P?? 0.05). LIRA treatment blocked the expression of IL ‐6 and TNF ‐α ( P?? 0.01), increased the formation of mineralization nodes ( P? ? 0.01), and enhanced the expression of ALP and Runx2 ( P?? 0.05). Conclusion LIRA can enhance the proliferation, migration, and osteogenic differentiation of hPDLC s and inhibit the inflammatory response. Thus, LIRA may have potential therapeutic use as an adjuvant treatment for human periodontitis, and this effect is independent of hypoglycemic activity.