首页> 外文期刊>Journal of molecular histology >Histomorphological, VEGF and TGF-beta immunoexpression changes in the diabetic rats' ovary and the potential amelioration following treatment with metformin and insulin
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Histomorphological, VEGF and TGF-beta immunoexpression changes in the diabetic rats' ovary and the potential amelioration following treatment with metformin and insulin

机译:糖尿病大鼠卵巢的组织形态,VEGF和TGF-β免疫表达变化及二甲双胍和胰岛素治疗后的潜在改善

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摘要

Diabetes mellitus (DM) affects the ovary by reducing the number and diameters of ovarian follicles and increasing atretic follicles. Follicular growth and diameters depend on VEGF production. Hyperglycemia causes ovarian stromal and follicular degeneration then fibrosis by activating TGF-beta. Insulin and metformin promote development of ovarian follicles and reduce atretic follicles. Therefore, the present study investigates the ovarian VEGF and TGF-beta immune-expression and its variations in diabetic, insulin and metformin-treated rats. Forty adult female albino rats were divided equally into four groups: control, diabetic (STZ-induced diabetes), diabetic metformin-treated group (100 mg/kg/day orally/eight weeks) and diabetic insulin-treated group (5 U insulin /day). Ovarian sections were stained with hematoxylin and eosin, Masson's trichrome, immunohistochemistry for VEGF and TGF-beta. The diabetic group showed noticeable atrophic and degenerative changes in cortex and medulla as well as increased density and distribution of the collagenous fibers. The number and diameter of primary, secondary and tertiary follicles were decreased. However, the number of atretic follicles and corpus luteum was increased. Significant decrease in the surface area percentage of VEGF immuno-expression and significant increase in TGF-beta immuno-expression surface area percentage were detected. By treating animals with metformin and insulin, there was restoration of the ovarian histological structure more or less as in control. DM negatively affects the histological and morphometric parameters of ovaries. Furthermore, insulin showed more beneficial effects than metformin in hindering these complications by modifying the expression of VEGF and TGF-beta.
机译:糖尿病(DM)通过减少卵巢卵泡的数量和直径和增加胃卵泡来影响卵巢。卵泡生长和直径取决于VEGF生产。通过激活TGF-β导致卵巢基质和卵泡变性导致卵巢纤维化。胰岛素和二甲双胍促进卵巢卵泡的发育并减少娱乐卵泡。因此,本研究研究了卵巢VEGF和TGF-β免疫表达及其糖尿病,胰岛素和二甲双胍治疗大鼠的变化。将四十个成年雌性白化大鼠分别分为四组:对照,糖尿病(STZ诱导糖尿病),糖尿病二甲双胍治疗组(100mg / kg /天口服/八周)和糖尿病胰岛素治疗组(5u胰岛素/日)。卵巢切片用苏木精和曙红,马隆的血管素,VEGF和TGF-β的免疫组化。糖尿病组在皮质和髓质中显示出明显的萎缩和退行性变化以及胶原纤维的密度和分布增加。初级,二级和三级卵泡的数量和直径降低。然而,娱乐卵泡和语料树脂的数量增加。检测到VEGF免疫表达的表面积百分比和TGF-β免疫表达表面面积面积百分比的显着降低。通过用二甲双胍和胰岛素治疗动物,或多或少地恢复卵巢组织学结构。 DM对卵巢的组织学和形态学参数产生负面影响。此外,通过改变VEGF和TGF-β的表达,胰岛素比妨碍这些并发症的二甲双胍显示更有益的效果。

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