Development and validation of a mouse-based primary screening method for testing relative allergenicity of proteins from different wheat genotypes

摘要

Background: Wheat allergy is a major food allergy that has reached significant levels of global public health concern. Potential variation in allergenicity among different wheat genotypes is not well studied at present largely due to the unavailability of validated methods. Here, we developed and validated a novel mouse-based primary screening method for this purpose.