Study and identification of Insulin-like Growth Factor-I gene polymorphisms in Zel sheep population.

摘要

Problem statement: The IGFs play an important role in regulating somatic growth according to nutritional conditions. Polymorphisms of IGF gene are reported to be significantly associated with many traits. Approach: In these study 142 samples of DNA from Zel sheep were used for detecting the polymorphisms of promoter region of the Insulin-like Growth Factor-1 (IGF-1) gene. Therefore DNA extracted from blood using Salting-out procedure. Results: Primers were supplied for amplifying the specific segment. Polymerase Chain Reaction (PCR) accomplished after finding the best condition to do the reaction. The specific segment amplified well. SSCP and RFLP markers were used for detecting the polymorphisms of the segment. For SSCP analysis PCR-product was denatured and dilution was loaded onto a 12% polyacrylamide gel. RFLP analysis was performed by incubating of PCR product by HaeII restriction enzyme at 37 degrees C for 4 h. Gels were visualized using a 3.5% agarose gel that contained ethidium bromide. The polymorphisms were the same when both methods were examined. Evaluation of result revealed 2 alleles and 3 genotypes. The alleles were A and B and their frequencies was 0.71 and 0.29 respectively. The genotyped named AA, AB and BB with the frequencies of 0.47, 0.47 and 0.06 respectively. Conclusion/Recommendations: The data were analyzed for Genetic variation statistics using PopGene32 software and no deviation from Hardy-Weinberg equilibrium was observed in this study. finding the relation between genotype which is found by genetic markers with growth traits, live weight and carcass weight will be investigate by the IGFs genes.