Ferric citrate, nitrate, saponin and their combinations affect in vitro ruminal fermentation, production of sulphide and methane and abundance of select microbial populations

摘要

Aims This study investigated the effects of ferric citrate, nitrate and saponin, both individually and in combination, on sulphidogenesis, methanogenesis, rumen fermentation and abundances of select microbial populations using in vitro rumen cultures. Methods and Results Ferric citrate (50 mg l(-1)), Quillaja saponin (0 center dot 6 g l(-1)) and sodium nitrate (5 mmol l(-1)) were used in in vitro ruminal fermentation. Ferric citrate alone, its combination with saponin and/or nitrate lowered the aqueous sulphide concentration and total sulphide production. Methane production was suppressed by nitrate alone (by up to 32 center dot 92%), its combination with saponin (25 center dot 04%) and with both saponins with nitrate (25 center dot 92%). None of the treatments adversely affected feed digestion or rumen fermentation. The population of sulphate-reducing bacteria was increased by nitrate and saponin individually, while that of total Archaea was decreased by nitrate alone and the combination of the three inhibitors. Conclusions Nitrate and its combination with saponin or both ferric citrate and saponin substantially decreased methane production. Most importantly, the decreased methane production was not at the expense of feed digestion or fermentation. Sulphidogenesis from the sulphate present in the high-sulphur diets can be suppressed competitively by ferric citrate, although it was elevated by saponin and nitrate. Significance and Impact of the Study The results of this study demonstrated that combinations of certain methane inhibitors, which have different mechanisms of antimethanogenic actions or inhibit different guilds of microbes involved in methane production and sulphate reduction, can be more effective and practical than individual inhibitors, not only in mitigating enteric methane emission but also in lowering the risk of sulphur-associated polioencephalomalacia in feedlot cattle fed high sulphur diets.