Analysis of Molecular Variance and Population Structure inSouthern Indian Finger Millet Genotypes Using ThreeDifferent Molecular Markers

摘要

The genetic relationship among 42 genotypes of finger millet collected from different geographical regions of southernIndia was investigated using random amplified polymorphic DNA (RAPD), inter simple sequence repeats (ISSR), and simplesequence repeats (SSR) markers. Ten RAPD primers produced 111 polymorphic bands. Five ISSR primers produced a totalof 61 bands. Of these, 23 bands were polymorphic. The RAPD and ISSR fingerprints revealed 71.3 and 37.4% polymorphicbanding patterns, respectively. Thirty-six SSR primers yielded 83 scorable alleles in which 62 were found to be polymorphic.Out of 36 SSR primers used, 14 primers (46.6%) produced polymorphic bands. The SSR primer UGEP7 produced amaximum number of six alleles. Mean polymorphic information content (PIC) of RAPD, ISSR and SSR were 0.44, 0.28, and0.14, respectively. Molecular variances among the population were 2, 11, and 1% for RAPD, ISSR, and SSR markers,respectively. SSR produced 99% molecular variance within individuals. RAPD and ISSR markers produced a low level ofmolecular variance within individuals. The STRUCTURE (model-based program) analysis revealed that the 42 finger milletgenotypes could be divided into a maximum of four subpopulations. Based on the Bayesian statistics, each RAPD and SSRmarker produced three subpopulations (K=3), while ISSR marker showed four subpopulations (K=4). This study revealedthat RAPD and SSR markers could narrow down the analysis of population structure and it may form the basis for fingermillet breeding and improvement programs in the future.