Determination of human insulin in dog plasma by a selective' liquid, chromatography-tandem mass spectrometry method:. Application to a pharmacokinetic study

摘要

A simple, sensitive and selective LC-MS/MS method for quantitative analysis of human insulin was developed) and validated in dog plasma. Insulin glargine was used as the internal standard. After a simple step of solid-phase extraction, the chromatographic separation of human insulin was achieved by using InertSustain Bio C18 column with a mobile phase of acetonitrile containing 1% formic acid (A) water containing 1% formic acid (B). The detection was performed by positive ion electrospray ionization in multiple-reaction monitoring (MRM) mode. Good linearity was observed in the concentration range of 1-1000 IU/mL (r2 0.99), and the lower limit of quantification was 1 iilU/mL (equal to 38.46 pg/mL). The intra-and inter-day precision (expressed as relative standard deviation, RSD) of human insulin were 512.1% and 513.0%, respectively, and the accuracy (expressed as relative erior, RE) was in the range of-7.23-11.9%. The recovery and matrix effect were both within acceptable limits. This method was successfully applied for the phannicokinetic study of human insulin in dogs ' after subcutaneous administration.