A rapid and sensitive supercritical fluid chromatography/tandem mass spectrometry method for detection of ezetimibein dog plasma and its application in pharmacokinetic studies

摘要

Highlights?We determined ezetimibe in dog plasma by a rapid, high-selective and sensitive SFC–MS/MS.?Ezetimibe-glucuronide should be hydrolyzed to the original drug by the β-Glucuronidase.?The total free ezetimibe will be detected by MS after liquid-liquid extraction.AbstractThe aim of this study is to develop and validate a rapid, high-selective and sensitive supercritical fluid chromatography/tandem mass spectrometry (SFC–MS/MS) with a multiple reactions monitoring (MRM) mode method for the detection of ezetimibe in dog plasma. Several conditions were optimized systematically as follows: lipid–lipid extraction (LLE) performances were used to extract analytes from dog plasma; an ACQUITY HSS C18SB (1.8?μm, 3.0?×?100?mm) column was employed to separate the target compounds; the triple-quadrupole mass spectrometry equipped with electrospray ionization (ESI) source was applied to detect ezetimibe. The method, which required a relatively small volume of plasma (100?μL), was