...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of chromatography, B. Analytical technologies in the biomedical and life sciences >Quantification of glycocholic acid in human serum by stable isotope dilution ultra performance liquid chromatography electrospray ionization tandem mass spectrometry
【24h】

Quantification of glycocholic acid in human serum by stable isotope dilution ultra performance liquid chromatography electrospray ionization tandem mass spectrometry

机译：通过稳定同位素稀释量血清甘油酸中的甘油酸的定量超现液相色谱电器电离电离串联质谱法

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Highlights?A stable isotope dilution UPLC-ESI–MS/MS method for the detection of glycocholic acid was developed.?The method was successfully applied to quantify glycocholic acid in human serum.?32 healthy volunteers, 10 patients with hepatocellular carcinoma and 14 patients with other cancers were recruited.?Levels of glycocholic acid in patients with hepatocellular carcinoma were significantly increased.AbstractA rapid, accurate and sensitive stable isotope dilution ultra performance liquidchromatography electrospray ionization tandem mass spectrometry (ID-UPLC-ESI–MS/MS) method for the determination of glycocholic acid (GCA) in human serum was developed and validated. Serum samples were spiked with D5-glycocholic acid and then pretreated with protein precipitation. The analysis was performed on a Waters BEH C18 column (100 mm×2.1mm, 1.7μm), followed by ESI–MS/MS dete
机译：<！[cdata [ 突出显示 ？ 开发了一种稳定的同位素稀释UPLC-ESI-MS / MS检测甘油酸的方法。 ？ 方法已成功应用该方法在人血清中量化甘油酸。 ？ 32个健康的志愿者，10名患有肝细胞癌和14名其他癌症患者的患者。 ？ 肝细胞癌患者的甘油酸水平显着增加。 抽象 快速，准确，敏感的稳定同位素稀释超级性能液晶电离电离串联开发并验证了用于测定人血清中甘油酸（GCA）的质谱（ID-UPLC-ESI-MS / MS）方法。用D 5 -glyCOCOLIC酸，然后用蛋白质沉淀预处理血清样品。该分析在Waters BEH C18柱上进行（100mm×2.1mm，1.7μm），然后是ESI-MS / MS DETE

著录项

  • 来源
  • 作者

    Cheng Guo; Cong Xie; Peili Ding; Guangming Qin; Weimin Mo; Xiaoji Cao; Shu Zheng;

  • 作者单位

    Cancer Institute (Key Laboratory of Cancer Prevention and Intervention China National Ministry of Education) The Second Affiliated Hospital Zhejiang University School of Medicine;

    Cancer Institute (Key Laboratory of Cancer Prevention and Intervention China National Ministry of Education) The Second Affiliated Hospital Zhejiang University School of Medicine;

    Cancer Institute (Key Laboratory of Cancer Prevention and Intervention China National Ministry of Education) The Second Affiliated Hospital Zhejiang University School of Medicine;

    Department of Laboratory The Second Affiliated Hospital Zhejiang University School of Medicine;

    College of Chemical Engineering Zhejiang University of Technology;

    College of Chemical Engineering Zhejiang University of Technology;

    Cancer Institute (Key Laboratory of Cancer Prevention and Intervention China National Ministry of Education) The Second Affiliated Hospital Zhejiang University School of Medicine;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物化学技术;
  • 关键词

    ID-UPLC-ESI–MS/MS; Glycocholic acid; Serum; Hepatocellular carcinoma; Biomarker;

    机译：ID-UPLC-ESI-MS / MS;甘油酸;血清;肝细胞癌;生物标志物;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号