In Vitro Biocidal Actions of Rhus verniciflua Bark Extract Wrapped Gold Nanoballs Against Biofilm-Forming Food-Borne Bacterial Pathogens

摘要

The research for innovative antibiofilm drugs is essential due to the increased microbial resistance to antibiotics presently in use. Now a day's natural products wrapped nano biomaterial play an important role in the field of medicine. The aqueous bark extracts of Rhus verniciflua was used to synthesize gold nanoparticles (Rh-AuNPs) by green method. The synthesized nanoparticles were characterized (optical property, crystallinity, functional groups, size, shape and purity) by UV-vis spectroscopy (UV), X-ray diffraction (XRD), Fourier transform infrared (FTIR), Field emission transmission electron microscopy (FE-TEM) with Energy dispersive X-ray (EDX). An effective antioxidant activity was observed at 100 mu g/ml of Rh-AuNPs. The acridine orange, live and dead (PI-SYTO-9) staining assay using confocal laser scanning microscopic, results evidanced that the Rh-AuNPs (100 mu g/ml) was effectively inhibited the biofilms of Escherichia coli and Staphylococcus aureus. In addition, Rh-AuNPs also significantly inhibited the cell surface hydrophobicity of both E. coli and S. aureus at 100 mu g/ml. This study reports antibacterial potency of Rh-AuNPs and that have significant therapeutic properties.