Development of the cerebellum in turbot ( Psetta maxima ): Analysis of cell proliferation and distribution of calcium binding proteins

摘要

Highlights ? Metamorphosis is a critical period for differentiation of the turbot mature cerebellum. ? Ontogeny of neurons and cerebellar connectivity is evidenced by expression of CaBPs. ? Cytoarchitecture of proliferative zones may reflect inner migratory pathways in the cerebellum. Abstract The morphogenesis, cell proliferation and neuronal differentiation of the turbot ( Psetta maxima ) cerebellum has been studied using conventional histological techniques and immunohistochemical methods for proliferating cell nuclear antigen and calcium binding proteins. As in other vertebrates, the cerebellar anlage emerges as proliferative plates of neural tissue during the embryonic period. The anlage of the cerebellum persists without morphological changes until the end of the larval life when the mantle zone is differentiated. The major ontogenetic changesthat drive the formation of the cerebellar subdivisions begin in late premetamorphic larvae when cerebellar plates growth and merge medially. This transformation is accomplished by the reorganization of proliferative zones as well as by the onset of cell differentiation. The cerebellum becomes fully differentiated during metamorphosis when parvalbumin and calretinin were detected in Purkinje and eurydendroid cells. Sustained proliferation is maintained in all subdivisions of the cerebellum and this support the robust growth of this part of the brain that takes place during the metamorphic and juvenile periods.The location and histological organization of the proliferative activity in the turbot mature cerebellum are described and their functional significance was analyzed in light of the information available for other teleosts.