A scs~(ae) gene on chromosome ID alters the expression of a gametocidal (scs~(spt)) gene in a T2BL.2S chromosome

摘要

A species cytoplasm specific gene (scs~(ae)) on the long-arm of chromosome 1D (1DL) from common wheat (Triticum aestivum L.) produced compatibility of the nuclear genome of durum wheat (T. turgidum L.) with the cytoplasm of D- or M-genome Aegilops species, including an altered cytoplasm of Ae. longissima (lo). Similarly, a 29-chromosome durum line with Ae. uniaristata (uri) cytoplasm and a un-telocentric homoeologous to group 4 chromosomes, a (lo) durum line with scs~(ti) in the long-arm of chromosome 1A from T. timopheevi, and a (Ae. ventricosa) durum [(vent) line] with scs~(spt) in T2BL.2S translocation chromosome having 2S from Ae. speltoides were compatible but male-sterile. Our objective was to determine the transmission of female gametes withscs~(ae) versus those with scs~(ti), scs~(un), or scs~(spt). We crossed (lo) SCS~(ti), (un) scs~(un), and (vent) scs~(spt) durum lines to 1D(1A) and 1D(1B) disomic-substitution lines of Langdon durum (LDN DS) and crossed hybrid progeny to the corresponding paternal LDN DS lines as recurrent male parents. The resulting alloplasmic LDN DS lines were male sterile and when crossed to paternal LDN DS lines; (lo) and (uri) DS lines had complete seed-set and 14 closed chromosome pairs, while (vent) DS lines had partial seed-set, 13 closed chromosome pairs and 1 open pair at the meiotic metaphase I in the pollen mother cells. The results showed that scs~(ae) replaced scs(ti) or scs~(un) in the (lo) and (uri) DS lines, that 2S did not pair with 2BS, and that scs~(spt) was expressed as a gametocidal (Gc) gene but did not produce chromosomal structural abnormalities in the (vent) 1D(1A) and 1D(1B) DS lines having scs~(ae) in chromosome ID.