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Successive ethanol–water fractionation of enzymatic hydrolysis lignin to concentrate its antimicrobial activity

机译：酶水解木质素的连续乙醇水分馏浓缩其抗微生物活性

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Abstract <section xml:id="jctb5656-sec-0001" numbered="no"> <title type="main" xml:lang="de">BACKGROUND >With the emergence of lignocellulose refining, there is an increasing interest in exploring the valuable applications for lignin due to its abundant and underused features. Originating from the physiological function of lignin in plants, this work attempted to evaluate the antimicrobial potential of enzymatic hydrolysis lignin (EHL) from a bioethanol‐derived biorefinery. </section> <section xml:id="jctb5656-sec-0002" numbered="no"> <title type="main">RESULTS > Successive ethanol–water dissolution was employed to fractionate EHL and the antimicrobial activities of three obtained lignin fractions (F1, F2 and F3) were compared by the agar diffusion method and minimum inhibitory concentration (MIC) assays. Interestingly, the results showed that the antimicrobial activities of EHL were centralized in F1 which exhibited obviously growth inhibitory effects against two Gram‐positive ( <fc> <fi>Staphylococcus aureus</fi> </fc> and <fi>Bacillus subtilis</fi> ) and two Gram‐negative ( <fc> <fi>Escherichia coli</fi> </fc> and <fi>Salmonella enterica</fi> ) bacteria with MICs of 1, 2, 2 and 2?mg?mL ?1 , respectively. The further characterization suggested that the better solubility and higher phenolic hydroxyl content of F1 caused by lower molecular weight were possibly responsible for the improvement of antimicrobial performance. </section> <section xml:id="jctb5656-sec-0003" numbered="no"> <title type="main">CONCLUSIONS >Consequently, this work </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract XMLNS =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> <section XML：ID =“JCTB5656-SEC-0001”编号=“否”> <标题类型=“main”XML：Lang =“de”>背景</标题> >随着木质纤维素炼油的出现，越来越兴趣探索木质素的宝贵应用因其丰富和未充分利用的特征。来自植物中木质素的生理功能，这项工作试图评估酶水解木质素（EHL）的生物乙醇衍生的生物颗粒的抗微生物潜力。</ p> </段> <截面XML：ID =“JCTB5656-SEC -0002“编号=”否“> <标题类型=”main“>结果</标题> >逐次乙醇 - 水溶解将用于分馏EHL和三种获得的木质素级分的抗微生物活性（F1，F2和F3通过琼脂扩散方法和最小抑制浓度（MIC）测定进行比较。有趣的是，结果表明，EHL的抗微生物活性在F1中集中，其表现出对两种革兰阳性（<FC> <FI>金黄色葡萄球菌</ FI> </ FC>和<FI> Bacillus枯草芽孢杆菌的生长抑制作用/ fi>）和两种革兰阴性（<fc> <fi>大肠杆菌</ fi> </ fc>和<fi> salmonella enterica </ fi>）细菌，具有1,2,2和2？mg ？分别是ml ？1 </ sup>。进一步表征表明，由较低分子量引起的F1的溶解性和较高的酚羟基含量可能负责改善抗微生物性能。 </ p> </ section> <第XML：ID =“JCTB5656-SEC-0003”编号=“否”> <标题类型=“main”>结论</ title> >因此，这项工作 </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-33908/'>《Journal of Chemical Technology & Biotechnology》</a> <b style="margin: 0 2px;">|</b><span>2018年第10期</span><b style="margin: 0 2px;">|</b><span>共11页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wang Guanhua&option=202" target="_blank" rel="nofollow">Wang Guanhua;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Xia Yue&option=202" target="_blank" rel="nofollow">Xia Yue;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Liang Benke&option=202" target="_blank" rel="nofollow">Liang Benke;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sui Wenjie&option=202" target="_blank" rel="nofollow">Sui Wenjie;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Si Chuanling&option=202" target="_blank" rel="nofollow">Si Chuanling;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Tianjin Key Laboratory of Pulp and Paper College of Paper Making Science and TechnologyTianjin University of Science and TechnologyTianjin China;</p> <p>Tianjin Key Laboratory of Pulp and Paper College of Paper Making Science and TechnologyTianjin University of Science and TechnologyTianjin China;</p> <p>Tianjin Key Laboratory of Pulp and Paper College of Paper Making Science and TechnologyTianjin University of Science and TechnologyTianjin China;</p> <p>Key Laboratory of Food Nutrition and Safety (Tianjin University of Science &</p> <p>Technology)Ministry of EducationTianjin China;</p> <p>Tianjin Key Laboratory of Pulp and Paper College of Paper Making Science and TechnologyTianjin University of Science and TechnologyTianjin China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/180.html" title="生物工程学（生物技术）">生物工程学（生物技术）;</a><a href="https://www.zhangqiaokeyan.com/clc/20827.html" title="生物化学过程">生物化学过程;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=lignin fractionation&option=203" rel="nofollow">lignin fractionation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=antimicrobial activity&option=203" rel="nofollow">antimicrobial activity;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=molecular weight&option=203" rel="nofollow">molecular weight;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=characterization&option=203" rel="nofollow">characterization;</a> </p> <div class="translation"> 机译：木质素分馏;抗微生物活性;分子量;表征; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025423235.html">Successive ethanol–water fractionation of enzymatic hydrolysis lignin to concentrate its antimicrobial activity</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wang Guanhua&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Wang Guanhua,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Xia Yue&option=202" target="_blank" rel="nofollow" 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