A novel food‐grade lactococcal expression system and its use for secretion and delivery of an oral vaccine antigen

Zhang Rongguang; Qiao Dan; Peng Xiaoyan; Duan Guangcai; Shi Qingfeng; Zhang Linghan; Wang Chen; Liang Wenjuan; Chen Shuaiyin; Fan Qingtang

摘要

Abstract <section xml:id="jctb5536-sec-0001" numbered="no"> <title type="main" xml:lang="de">BACKGROUND >Food‐grade bacterial expression systems are relatively rare, and increasing evidence indicates that subcellular location of antigens in bacterial vector vaccines may markedly affect the immune efficacy. </section> <section xml:id="jctb5536-sec-0002" numbered="no"> <title type="main">RESULTS > This study developed a novel food‐grade secretory expression system for heterologous protein production and oral vaccine delivery. Furthermore, by using the expression system, an engineered <fi>L. lactis</fi> strain secreting <fi>H. pylori</fi> UreB was constructed, and used to vaccinate SPF BALB/c mice. As results, the UreB expressed in <fi>L. lactis</fi> was detected in both cell lysates and culture supernatant of the engineered strain, constituting roughly 50% of the culture supernatant proteins, and recognized by mouse anti‐ <fi>H. pylori</fi> sera. Oral vaccination with the engineered <fi>L. lactis</fi> produced a significantly elevated anti‐UreB serum antibody level in mice ( <fi>P</fi> ??0.05). </section> <section xml:id="jctb5536-sec-0003" numbered="no"> <title type="main">CONCLUSION > These data show a novel food‐grade <fi>L. lactis</fi> secretory expression system, which may have distinct potential impact on edible and medicinal protein production and oral vaccine development. Moreover, this is the first report on secretory expression of a <fi>H. pylori</fi> antigen via using a food‐grade lactococcal expression </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract XMLNS =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> <section XML：ID =“JCTB5536-SEC-0001”编号=“否”> <标题类型=“main”XML：Lang =“de”>背景</标题> >食物级细菌表达系统相对罕见，越来越多的证据表明细菌载体疫苗中抗原的亚细胞位置可以显着影响免疫功效。</ p> </ section> <secto XML：ID =“JCTB5536-SEC-0002”编号=“否”> <标题类型=“main “>结果</标题> >本研究开发了一种新型食品级分泌表达系统，用于异源蛋白质生产和口服疫苗递送。此外，通过使用表达式系统，工程化<fi> l。 Lactis </ Fi>应变分泌<FI> H.幽门螺杆菌</ Fi> UreB构建，并用于疫苗接种SPF BALB / C小鼠。结果，ureb在<fi> l中表达。在工程菌株的细胞裂解物和培养上清液中检测到乳酸乳酸乳裂，构成大约50％的培养上清蛋白，并通过小鼠抗体H识别。幽门螺杆菌</ fi>血清。用工程化的口腔疫苗接种<fi> l。 Lactis </ Fi>在小鼠中产生显着升高的抗UREB血清抗体水平（<fi> p </ fi>β.<0.05）。 </ p> </ section> <section XML：ID =“JCTB5536-SEC-0003”编号=“否”> <标题类型=“main”>结论</ title> >这些数据显示了一种新颖的食物 - 级别<fi> l。 Lactis </ Fi>分泌表达系统，这可能对食用和药用蛋白质产生和口腔疫苗发育具有明显的潜在影响。此外，这是<fi> h的分泌表达的第一个报告。 Pylori </ Fi>抗原通过使用食物级乳球菌表达 </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-33908/'>《Journal of Chemical Technology & Biotechnology》</a> <b style="margin: 0 2px;">|</b><span>2018年第6期</span><b style="margin: 0 2px;">|</b><span>共6页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Rongguang&option=202" target="_blank" rel="nofollow">Zhang Rongguang;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Qiao Dan&option=202" target="_blank" rel="nofollow">Qiao Dan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Peng Xiaoyan&option=202" target="_blank" rel="nofollow">Peng Xiaoyan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Duan Guangcai&option=202" target="_blank" rel="nofollow">Duan Guangcai;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Shi Qingfeng&option=202" target="_blank" rel="nofollow">Shi Qingfeng;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Linghan&option=202" target="_blank" rel="nofollow">Zhang Linghan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wang Chen&option=202" target="_blank" rel="nofollow">Wang Chen;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Liang Wenjuan&option=202" target="_blank" rel="nofollow">Liang Wenjuan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Chen Shuaiyin&option=202" target="_blank" rel="nofollow">Chen Shuaiyin;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fan Qingtang&option=202" target="_blank" rel="nofollow">Fan Qingtang;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Epidemiology and Statistics College of Public HealthZhengzhou UniversityZhengzhou China;</p> <p>Department of Epidemiology and Statistics College of Public HealthZhengzhou UniversityZhengzhou China;</p> <p>Department of Epidemiology and Statistics College of Public HealthZhengzhou UniversityZhengzhou China;</p> <p>Department of Epidemiology and Statistics College of Public HealthZhengzhou UniversityZhengzhou China;</p> <p>Department of Epidemiology and Statistics College of Public HealthZhengzhou UniversityZhengzhou China;</p> <p>Department of Clinical MedicineZhengzhou UniversityZhengzhou China;</p> <p>Department of Epidemiology and Statistics College of Public HealthZhengzhou UniversityZhengzhou China;</p> <p>Department of Epidemiology and Statistics College of Public HealthZhengzhou UniversityZhengzhou China;</p> <p>Department of Epidemiology and Statistics College of Public HealthZhengzhou UniversityZhengzhou China;</p> <p>Department of Epidemiology and Statistics College of Public HealthZhengzhou UniversityZhengzhou China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/180.html" title="生物工程学（生物技术）">生物工程学（生物技术）;</a><a href="https://www.zhangqiaokeyan.com/clc/20827.html" title="生物化学过程">生物化学过程;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lactococcus lactis&option=203" rel="nofollow">Lactococcus lactis;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=food‐grade&option=203" rel="nofollow">food‐grade;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=secretory expression&option=203" rel="nofollow">secretory expression;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=oral vaccine&option=203" rel="nofollow">oral vaccine;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Helicobacter pylori&option=203" rel="nofollow">Helicobacter pylori;</a> </p> <div class="translation"> 机译：乳球菌乳酸;食品级;分泌表达;口腔疫苗;幽门螺杆菌; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025422887.html">A novel food‐grade lactococcal expression system and its use for secretion and delivery of an oral vaccine antigen</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Rongguang&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Zhang Rongguang,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Qiao Dan&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Qiao 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K.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Simon, J. K.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext= Maciel, M.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> Maciel, M.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext= Weld, E. D.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> Weld, E. D.,</a> <span>2011</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：抗原特异性lga B记忆细胞对志愿者中引起的志贺氏菌抗原的反应，所述志愿者用活的减毒志贺氏菌2a口服疫苗候选物免疫</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_detail_thesis/0201294986077.html">乳酸杆菌作为一种新型活疫苗抗原递送载体</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=庾庆华&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 庾庆华</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=杨倩&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,杨倩</a> <span> <a href="/journal-cn-56766/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 世界华人消化杂志 </a> </span> <span> . 2005</span><span>,第18期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-nantong-university-medical-sciences_thesis/0201245447106.html">乳酸乳球菌食品级表面表达系统建立和报告基因检测</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=庄重&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 庄重</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=季莉&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,季莉</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=徐莹莹&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,徐莹莹</a> <span> <a href="/journal-cn-6533/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 南通大学学报（医学版） </a> </span> <span> . 2015</span><span>,第004期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-cn_detail_thesis/0201298308947.html">重组植物甜蛋白马宾灵的食品级乳酸乳球菌诱导表达系统的构建</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=顾文亮&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 顾文亮</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=夏启玉&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,夏启玉</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=姚晶&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,姚晶</a> <span> <a href="/journal-cn-56720/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中国农学通报 </a> </span> <span> . 2012</span><span>,第30期</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-cn_acta-microbiologica-sinica_thesis/0201255181188.html">乳酸乳球菌食品级诱导表达系统的构建及异源蛋白的表达</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=徐波&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 徐波</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=曹郁生&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,曹郁生</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈燕&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,陈燕</a> <span> <a href="/journal-cn-5094/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 微生物学报 </a> </span> <span> . 2007</span><span>,第004期</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-journal-cn_adhesion_thesis/0201296078031.html">新型纳米粒子或可用于疫苗安全递送</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . </a> <span> <a href="/journal-cn-13389/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 粘接 </a> </span> <span> . 2011</span><span>,第4期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-1547_thesis/02022200078.html">一种新型抗胃泌素17疫苗的体液免疫原性与抗原性研究</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=HE Qing&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . HE Qing</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=贺庆&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,贺庆</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=GAO Meng&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,GAO Meng</a> <span> <a href="/conference-cn-1547/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 2015中国生物制品年会暨第十五次全国生物制品学术研讨会 </a> <span> <span> . 2015</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020315848815.html">新型乳酸杆菌递送载体的筛选及金黄色葡萄球菌口服疫苗的研究</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=吕月清&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 吕月清</a> <span> . 2020</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06120100972976.html">新型格氏乳球菌噬菌体LAC-GAP-3及其用于抑制格氏乳球菌细菌增殖的用途</a> <b>[P]</b> . <span> 中国专利： CN110023492A </span> <span> . 2019-07-16</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120113294122.html">用于递送有效治疗上皮屏障功能障碍的蛋白质的乳酸乳球菌表达系统</a> <b>[P]</b> . <span> 中国专利： CN113347983A </span> <span> . 2021-09-03</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130435337879.html">mixtures of antigenic peptides or polypeptide, antigenic peptides, antigenic polypeptide, antigenic mixtures, lipopeptide, myxotope, lipomixotope, conjugate, immunogenic compositions, malaria vaccine, use of a mixture, polyclonal serum, recombinant human antibody, uses of a composition, methods and kits for the in vitro diagnosis of malaria, nucleotide sequences, cloning vector and / or recombinant expression, use of a recombinant host cell and DNA expression vector</a> <b>[P]</b> . <span> 外国专利：  BRPI0417286A </span> <span> . 2007-04-10</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：抗原性肽或多肽的混合物，抗原性肽，抗原性多肽，抗原性混合物，脂肽，黏附体，脂质混合体，缀合物，免疫原性组合物，疟疾疫苗，混合物的使用，多克隆血清，重组人抗体，组合物的用途，方法和试剂盒用于体外诊断疟疾，核苷酸序列，克隆载体和/或重组表达，使用重组宿主细胞和DNA表达载体 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130432700730.html">Oral vaccine and new application of yeast capable of antigen expression</a> <b>[P]</b> . <span> 外国专利：  PL199189B1 </span> <span> . 2008-08-29</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：口服疫苗及能表达抗原的酵母的新应用 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130442395730.html">ORAL VACCINE AND NEW APPLICATION OF YEAST CAPABLE OF ANTIGEN EXPRESSION</a> <b>[P]</b> . <span> 外国专利：  PL354719A1 </span> <span> . 2003-12-29</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：口腔疫苗及其在酵母中表达抗原的新应用 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704025422887','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" alt="关闭" 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