A rapid and simplified extraction method of erythromycin from fermentationbroth with bond elut C18 cartridge for analysis by HPLC

摘要

Solid phase extraction of erythromycin from fermentation broth is described in this paper. This method results in 85 percent recovery of erythromycin A with a batch to batch variability of less than 3 percent. Each column can be used twice with 2-3% loss of recovery. The columns are regenerated immediately after each extraction. In comparison with liquid liquid extraction, solid phase extracts are cleaner, the method is less time consuming and exposure to hazardous solvents is reduced.