A Novel Approach to Synthesis and Characterization of Silver Nanoparticles from Gorgonian Primnoa Spp. and Their Biomedical Approach: An In Vitro Studies

摘要

The gorgonian corals are a rich source of biologically active compounds which play important role in pharmacological and biomedical applications. This study was carried out invitro assessments of biomedical importance of silver nanoparticle synthesized Gorgonian Primnoa Spp. The Proximate composition of the crude Gorgonian Primnoa Spp. were studied protein carbohydrates and lipid. Further, bioactive fractions of Gorgonian Primnoa Spp. were identified using TLC and FTIR. The AgNPs were characterized using UV-Vis Spectroscopy, Scanning Electron Microscopy and Fourier Transform Infra Red Spectroscopy analysis. Furthermore these biologically synthesized nanoparticles were found to be highly effective against bacterial infection, inflammation and free radical scavenging activity were analyzed by using standard methods. In FTIR analysis, Primnoa Spp. extract showed various peaks such as Alcohols, Phenols, Nitriles, Alkanes, Aliphatic amines and Alkyl halides. The AgNPs synthesized Primnoa Spp. were characterized UV-vis analysis proved the wavelength 420 nm, SEM results showed that the particles size range of 11 nm. FTIR prediction indicated the presence of O-H stretch, free hydroxyl, O-H stretch, H-bonded, H-C=O:C-H stretch, C-H stretch, C-H bends, C-H rock, C-N stretch, C-Br stretch respectively. In hemolytic assay, the maximum activity 128 HU. In anti microbial assay, the highest susceptibility 25 mm and 15 mm was recorded in Staphylococcus aureus, Pseudomonas aeruginosa shows 24 mm and 17 mm, Shigella boydii shows 16 mm and 18 mm, Escherichia coli shows 8 mm and 12 mm and Vibrio sp. 6 mm and 10 mm respectively. The in vitro anti-inflammatory assay, AgNPs showed 68.92% and 72.1% of the protein denaturation inhibition and 68.9% μg/mL and 72.9% μg/mL were recorded in anti proteinase activity. The DPPH radical scavenging activity value is 51.3 and 56.8 μg/mL when compared to crude and AgNPs synthesized Primnoa Spp. This study provides the baseline information for synthe