Investigation on the Intercalation and Toxicity of 9-Aminoacridine in Zinc/Aluminium Layered Double Hydroxide Nanocomposites with Controlled Release Properties

摘要

Zinc/Aluminium layered double hydroxide (Zn/Al-LDH) nanocomposites were prepared by employing two different methods viz., co-precipitation and ion-exchange methods and labeled as ZAl-C and ZAl-E. 9-Aminoacridine (9-AA) is a potentive antitumor drug. However the presence of rapid metabolicdecomposition and its toxicity renders the potential drug to limit its usage. To overcome the dilemma, the drug was intercalated into the galleries of ZAl-C and ZAl-E. The resultant nanoformulations were labeled as AAZAl-C and AAZAl-E respectively. Powder-XRD, FT-IR, UV-Vis and elemental analysisreveals the intercalation of drugs into the LDH layers. The drug loading was estimated and found to be approximately 32.6% for AAZAl-C and 28.5% for AAZAl-E. The drug release rate from AAZAl-C and AAZAl-E were investigated at different pH (4.8 and 7.4) in phosphate buffer solution using kineticequations. The release of 9-AA from the interlayers of AAZAl-C and AAZAl-E were found to release at slow rate and governed by pseudo-second order kinetics. The toxicity of the drug (9-AA), AAZAl-C and AAZAl-E were assessed by brine shrimp toxicity (BST) test. The result shows that the toxicityof 9-AA was dramatically reduced when intercalated into the galleries of ZAl-C and ZAl-E. Our result finding emphasis that ZAl-C and ZAl-E found to be feasible for 9-AA to act as viable drug reservoir and drug delivery.