3O₄] Nano Particle to Promote the Properties for Industrial Applications]]>

摘要

Nano materials or nanotechnology is now one of the scintillating topic to investigate for modifying important properties of various bio molecules; is gaining much importance in the field of bio processing industries such as textiles, pharmaceuticals etc. Free enzyme used in bioprocessinghas many problems which include poor reusability, instability, poor recovery and high bio processing cost. Therefore, to overcome this we have synthesize magnetite nanoparticles through co-precipitation method. The properties and morphology of the prepared magnetite nanoparticles were characterizedusing scanning electron microscopy, FT-IR spectrophotometer and X-ray diffraction analysis. The α -amylase purified and characterize from Geobacillus sp Iso 5 was used to immobilize using amino functionalized (APTES) magnetite nanoparticles. Glutaraldehyde was used as across linker. These cross-linked enzyme aggregates were covalently attached to the magnetite nanoparticles. Further the enzymatic properties of the immobilized -amylase was investigated in contrast with free amylase. The immobilized thermostable -amylase has shown higher stability of pH andtemperature compare to free α -amylase. The enzyme was also reused after recovery of magnetic separation within 15 seconds. Immobilized hyper thermo stable enzyme could be used in textiles, fine-chemical synthesis, fermentation, pharmaceutical and other industries; performing reactionsat extreme conditions of high temperature and pH.