Alpha protons as NMR probes in deuterated proteins

摘要

We describe a new labeling method that allows for full protonation at the backbone H position, maintaining protein side chains with a high level of deuteration. We refer to the method as alpha proton exchange by transamination (-PET) since it relies on transaminase activity demonstrated here using Escherichia coli expression. We show that -PET labeling is particularly useful in improving structural characterization of solid proteins by introduction of an additional proton reporter, while eliminating many strong dipolar couplings. The approach benefits from the high sensitivity associated with 1.3mm samples, more abundant information including H resonances, and the narrow proton linewidths encountered for highly deuterated proteins. The labeling strategy solves amide proton exchange problems commonly encountered for membrane proteins when using perdeuteration and backexchange protocols, allowing access to alpha and all amide protons including those in exchange-protected regions. The incorporation of H protons provides new insights, as the close H-H and H-H-N contacts present in -sheets become accessible, improving the chance to determine the protein structure as compared with H-N-H-N contacts alone. Protonation of the H position higher than 90% is achieved for Ile, Leu, Phe, Tyr, Met, Val, Ala, Gln, Asn, Thr, Ser, Glu, Asp even though LAAO is only active at this degree for Ile, Leu, Phe, Tyr, Trp, Met. Additionally, the glycine methylene carbon is labeled preferentially with a single deuteron, allowing stereospecific assignment of glycine alpha protons. In solution, we show that the high deuteration level dramatically reduces R-2 relaxation rates, which is beneficial for the study of large proteins and protein dynamics. We demonstrate the method using two model systems, as well as a 32kDa membrane protein, hVDAC1, showing the applicability of the method to study membrane proteins.