Porous Structures on Titanium Surfaces: Biocompatibility and Effects on Osteogenic Activity In Vitro

摘要

In this work we synthesize porous structures, with varying pore diameters, on titanium surfaces by applying different electrochemical surface modification methods Among these methods, anodization is applied to synthesize titania nanotube (TNT)-based porous structures with varying pore diameters on titanium surfaces, and alkaline etching is used to synthesize micro-porous structures on titanium surfaces. In addition, both anodization and alkaline etching were applied to synthesize micro-nano porous structures with nanostructures on microtopography. We present preliminary in vitro data, including the test of Cell Counting Kit-8 (CCK-8), alkaline phosphatase (AKP), bone gla protein (BGP) and scanning electron microscopy (SEM), on their biocompatibility and effect on osteogenic activity, and provide an experimental basis for their development and clinical application as orthopedic implants and drug delivery systems. The results demonstrated that compared with micro- or nanostructures, the micro-nano composite porous structures had a greater ability to promote the mineralization of osteoblasts It is concluded that the micro-nano composite porous structures have good biocompatibility and osteogenic activity. Structures with nanopore diameters of 80-120 nm showed the best osteogenic activity.