Application Research of New Type of Zinc-Calcium-Phosphorus Membrane Pure Titanium Composite in Bone Tissue Regeneration

摘要

Objective: To investigate the application method of new type of zinc-calcium-phosphorus (ZCP) on pure titanium surface in bone tissue regeneration and antibacterial activity. Methods: In this study, we adopted the new type of ZCP on pure titanium surface as the support, and evaluated its role in bone tissue regeneration through the human osteoblast-like cell MG63 in vitro culture test. Results: The proliferation of MG63 in the ZCP group was greater than that in the pure titanium group, and ZCP MPTC materials were nontoxic. Cell adhesion on the surface of ZCP was faster. The cell alkaline phosphatase activity showed a significant difference between the ZCP group and the pure titanium group ( p < 0.05). The antibacterial ability of materials in the ZCP group was significantly stronger. Cells in the ZCP group was faster than that in the pure titanium group; moreover, the cells were larger and cell synapses were sturdier. Expression levels of osteogenesis genes (BSP, OPN, ALP, RUNX2, COL1A1, OSX and OC) and related proteins in the ZCP group were up-regulated. Conclusion: The new type of ZCP on pure titanium surface can promote cell adhesion, promote the growth of osteoblasts and finally promote the regeneration of bone tissue.