Poly(lactic-co-glycolic acid)/Basic Fibroblast Growth Factor Microspheres with Controllable Size by Coaxial Electrospray for Protein Drug Delivery

摘要

Core-shell microsphere is an ideal model for protein drug delivery which can be fabricated through coaxial electrospray. In this paper, Poly(lactic-co-glycolic acid) (PLGA) microspheres loaded with bFGF in the core part were successfully produced by coaxial electrospray techniques. The microspheres were prepared by different formulations and parameters. Four groups of microspheres were characterized by environmental scanning electron microscope (ESEM), transmission electron microscopy (TEM), encapsulation efficiency (EE) and in vitro bFGF release studies. The relationship between the size, thickness-to-radius (T/R) ratio, encapsulation efficiency and the protein drug release of microspheres was mainly discussed in this study. The PLGA-bFGF microspheres at a concentration of 5% and a voltage of 15 kV showed the smallest particle size and highest release rate. The microsphere was proved to be non-cytotoxic. The MTT assay showed that bFGF-loaded microspheres could promote fibroblasts cell proliferation and there was no cell cytotoxicity of PLGA microspheres in vitro. The results indicated that bFGF-loaded microspheres could be used as a sustained release protein drug delivery system in the fields of wound healing.