Improved Adhesion of Human Umbilical-Vein Endothelia Cells on Polystyrene Petri Dishes by the Formation of Functional Groups by Ar/H-2 Plasma Modification

摘要

Plasma approaches to improve biocompatibility have attracted much attention for researchers studying biomaterials. Using plasma is an effective method to control the physicochemical properties of surfaces. Control of physicochemical properties of surfaces is crucial for better cell adhesion in biological in vitro experiments. Though many researchers have studied cell adhesion on surfaces, the mechanism is still complicated, depending on the cell types, surface morphologies, and chemical properties. In this study, we discuss the relationship between improvement of the human umbilical-vein endothelial cell (HUVEC) adhesiveness .and surface chemical groups on surface-treated polystyrene Petri dishes HUVECs, which compose the interior surface of blood vessels or lymphatic vessels, do not adhere well on commercial unmodified polystyrene Petri dishes. Hence, the plasma processes were carried out by three methods, which were argon/hydrogen plasma surface treatment and functional film deposition from the ethylenediamine and the acetylene precursors. The HUVECs adhesiveness was dramatically improved on the argon/hydrogen plasma-treated Petri dish. We have characterized the surfaces of Petri dishes to analyze the factors for improving HUVEC adhesion on modified surfaces. Using X-ray photoelectron spectroscopy and water contact angle, we observed that the oxyl group (CO, COOH) was the influential factor affecting HUVEC adhesiveness on surfaces.