Polydatin inhibits cell proliferation and induces apoptosis in laryngeal cancer and HeLa cells via suppression of the PDGF/AKT signaling pathway

摘要

Abstract Polydatin (PD), a stilbene compound extracted from Polygonum cuspidatum , is suggested to possess anti‐cancer activities, including inhibition of cell proliferation, cell cycle arrest, and induction of apoptosis. The platelet‐derived growth factor (PDGF)/AKT signaling pathway plays complex roles in tumor suppression. However, the effect of PD on the PDGF/AKT signaling pathway in laryngeal cancer and HeLa cells has not been explored. MTT assay and flow cytometry showed that PD inhibited cell proliferation and induced apoptosis in Hep‐2 and AMC‐HN‐8 cells. Western blot analysis indicated that PD inhibited the expression levels of PDGF‐B and phosphorylated AKT (p‐AKT) in both cells. Treatment of PDGF‐B siRNA or PDGFR inhibitor found that after the PDGF signaling was inactivated, p‐AKT expression was significantly decreased in Hep‐2 cells. Tumor xenograft experiment in nude mice indicated PD significantly inhibited the growth of Hep‐2 cells in vivo . In conclusion, PD inhibited cell proliferation and induced apoptosis in laryngeal cancer and HeLa cells via inactivation of the PDGF/AKT signaling pathway.