In Vitro Cytocompatibility and Osteoinduction of Phosphorylated Chitosan with Osteoblasts

摘要

The cytocompatibility and osteoinduction of water-soluble p0hosphorylated chitosan (P-chitosan),that was modified by PO_3~92-) groups,with neonatal rat osteblasts in vitro was studied.To dientify the biocompatibility of P-chitosan with osteoblasts,the biofunctions of osteoblast cells,in the presence of three different concentrations of P-chitosan solutions as culture media and DMEM with 10% FBS as controls,were determiend.The osteoblast differences in the P-chitosan solutions andon chitosan films were also investigated with immunocytochemistry.It was found,based on the ALP activity and mineralization assay that water-soluble P-chitosan has excelent cytocompatibility compared to the chitosan without phosphorylated modification.P-chitosan concentrations as high as 2% had a significant influence on cytocompatibility an osteoinduction;one tissue (or derived product) caused a second undifferentiated tissue to differentiate into bone.Water-soluble P-chitosan could be a promising osteoinductive biomaterial for tissue engineering and orthopedic uses.