Effects of IFN-gamma on cell growth and the expression of ADAM33 gene in human embryonic lung Mrc-5 fibroblasts in vitro

摘要

Objective: To investigate the effects of interferon-gamma (IFN-gamma) on the proliferation and viability of human embryonic lung Mrc-5 fibroblasts in vitro and the expression of the A Disintegrin and Metalloprotease 33 (ADAM33) gene and to explore the mechanism of airway remodeling. Methods: Mrc-5 fibroblasts were sensitized with Dermatophagoides farinae 1 (Derf1) in vitro to mimic in vivo conditions observed in bronchial asthma. An inverted fluorescence microscope was used to observe changes in cell morphology before and after treatment. The viability of Mrc-5 cells was tested using the Cell Counting kit-8 (CCK8). Expression of the ADAM33 gene and protein in Mrc-5 cells was assessed using qPCR and Western blotting, respectively. Results: Different concentrations of Derf1 increased cell growth and the expression of the ADAM33 gene in Mrc-5 cells, and these changes were most obvious in the 10 mu g/ml group. In contrast, IFN-gamma decreased cell growth and the expression of the ADAM33 gene in both Mrc-5 cells and Derf1-induced Mrc-5 cells, and these changes were most obvious in the 10 ng/ml group. The negative effects of 10 ng/ml IFN-gamma were the most significant at 32 hours. Conclusions: Derf1-induced Mrc-5 cells successfully imitated the in vivo conditions observed in patients with asthma. IFN-gamma inhibited the proliferation and viability of Mrc-5 cells, and Derf1-induced Mrc-5 cells were more sensitive to IFN-gamma treatment. IFN-gamma treatment significantly downregulated the expression of the ADAM33 gene in a concentration- and time-dependent manner. IFN-gamma may participate in airway remodeling in asthma by regulating the expression of the ADAM33 gene.