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首页> 外文期刊>Journal of Applied Ichthyology >Identification of a sex-specific molecular marker in Salminus brasiliensis (Characiformes) based on SCAR marker
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Identification of a sex-specific molecular marker in Salminus brasiliensis (Characiformes) based on SCAR marker

机译:基于瘢痕标记的Salminus Brasiliensis(Characiformes)中的性特异性分子标记的鉴定

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摘要

Salminus brasiliensis, a characin regionally named dourado' (meaning goldfish), is the biggest characid fish, this species is cited in a list of fishes that are vulnerable. The goal of the present work was to try to identify, through AFLP technique to convert them into single locus markers (SCARs), in order to improve the sex identification in S.brasiliensis. Between possible sex-specific AFLP markers in this study, one male-specific were isolated (Les1) and converted into a SCAR marker. Les1 marker was confirmed sex-specific in all samples tested. A sex-specific DNA marker applicable to fish would be very useful for elucidating sex determination mechanism in fish.
机译:Salminus brasiliensis是一个区域名为Dourado'(意味着金鱼)的莎拉昔士,是最大的Characid鱼类,这个物种被引用在脆弱的鱼类清单中。 目前工作的目标是通过AFLP技术来识别它们将它们转换为单个轨迹标记(疤痕),以改善S.Brasiliensis中的性鉴定。 在本研究中可能的性别特异性AFLP标志物之间,分离一个男性特异性(LES1)并转化为瘢痕标记物。 在所有测试的样本中确认LES1标记被确认。 适用于鱼类的性别特异性DNA标记对于阐明鱼类中的性别测定机制非常有用。

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