SELECTION OF SUITABLE AND ECONOMIC SUBSTRATE FOR MASS PRODUCTION OF FUNGUS, BEAUVERIA BASSIANA

摘要

The experiment on mass production studies was conducted in the Entomology Laboratory, College of Agriculture, JNKVV, Jabalpur during Rabi 2013-14 on fourteen substrates to determine a suitable medium for growth and sporulation. During this period themaximum and minimum temperature of the laboratory were 39.55 ± 3.65 °C and 29.6 ± 6.4 °C, respectively while morning and evening relative humidity were 51 ± 19 % and 24.5 ± 8.5 %, respectively. The observations were recorded on 10th, 20thand 30th days after inoculation. The mean spore load recorded on solid and liquid substrates were 2.20 x 10 spores and 1.75 *107 spores, respectively. However, among the different solids substrates, broken grains recorded maximum spore load (2.61 x 107 spores), followed by husk (2.37 xio7 spores), bran (2.08 xio7 spores) and lowest on whole grains (1.78 x 10.7 spores), respectively. Among the different substrates evaluated significantly highest conidial count (4.77 x 10 spores/ml) was recorded on broken rice media having production cost of 3.44 followed by wheat husk (4.40 x 107 spores/ml), broken wheat (2.57 x 10 spores/ml) and wheat bran (2.20 x 107 spores/ml) having production cost of 3.16, 5.62 and 7.27, respectively.