Multiple exposure of Asian sea bass, (Lates calcarifer, Centropomidae) to clove oil: a histopathological study.

摘要

The following study describes the histopathological examination of Asian sea bass, Lates calcarifer, after being subjected to multiple exposure of 3, 6 and 9 ppm clove oil. The gills, hearts, livers and posterior kidneys of both exposed and non-exposed fish to clove oil were fixed in 10% formalin, processed and examined by light microscopy. The gills of fish exposed to 3 and 6 ppm did not differ from those of control fish. Fish, however, exposed to 9 ppm clove oil had necrosis of the epithelium lining the secondary lamellae of the distal region (tips) of the gill filament. Focal subepicardial haemorrhage was observed in the hearts of fish exposed to 9 ppm showing that slight increase in heart rate may have occurred. There were no alterations in the livers and posterior kidneys of exposed fish compared to control animals indicating the rapid elimination of clove oil.