LC-NMR Technique in the Analysis of Phytosterols in Natural Extracts

摘要

The ability of LC-NMR to detect simultaneously free and conjugated phytosterols in natural extracts was tested. The advantages and disadvantages of a gradient HPLC-NMR method were compared to the fast composition screening using SEC-NMR method. Fractions of free and conjugated phytosterolswere isolated and analyzed by isocraticHPLC-NMRmethods.Theresults of qualitative and quantitative analyses were in a good agreement with the literature data.