CONSTRUCTION OF PQQ-ENZYME MULTI-IMMOBILIZED ELECTRODES FOR ELECTROCATALTYIC REDUCTION OF CARBONYL COMPOUNDS

摘要

A thin poly(arylamine) (PAA) and poly(acrylic acid) (PAAc) layers-coated graphite felt (GF) electrode immobilizing all mediation components of pyrroloquinolinequinone (PQQ), diaphorase (Dp), oxidized nicotinamide adenine dinucleotide (NAD(+)) and alcohol dehydrogenase (ADH) to construct a complete bioelectrochemical reactor was prepared and applied to electrocatalytic reduction of carbonyl compounds in a phosphate buffer at constant potential of -0.65 V vs. Ag/AgCl. The carbonyl compounds were reduced to the corresponding alcohols with high current efficiency (97.2-100%) and high yield (97.4-100%), respectively.