IL-1beta in irrigation fluid and mRNA expression in synovial tissue of the knee joint as therapeutic markers of inflammation in collagen antibody-induced arthritis

摘要

Objective: We studied the relationship between the severity of inflammation and IL-1beta production and relativeexpression level of IL-1beta mRNA in irrigation fluid and synovial tissue obtained from the knee joint during the acute stage of amurine model of type II collagen antibody-induced arthritis (CAIA). This model is used to identify potential therapeutic markersfor treating rheumatoid arthritis.Methods: Irrigation fluid and synovium tissue were harvested from the knee joint of BALB/c mice in acute stage of CAIAinduction. The IL-1beta protein level was measured by enzyme-linked immunosorbent assay, and the relative expression level ofIL-1beta mRNA was analyzed by reverse transcription-polymerase chain reaction. Two investigators analyzed expression levelsand histopathological changes.Results: IL-1beta concentration was higher in irrigation fluid from the knee joint than in the serum in the acute stage of CAIA. Therelative expression level of IL-1beta mRNA was elevated in synovial tissue. Histopathological changes in the knee joint and footindicated similar severity.Conclusions: IL-1beta concentration in irrigation fluid and relative expression level of IL-1beta mRNA in the synovium have potentialas therapeutic markers in studying and treating CAIA.