Effect of c DMEM DMEM media containing Ectoine on human periodontal ligament mesenchymal stem cell survival and differentiation

摘要

Abstract Background/Aim Ectoine is an amino acid that can preserve osmotic balance and has more preservative activity than other osmoregulators. There are no published reports on the osmoregulators’ effects on viability or differentiation of dental stem cells. The aim of this study was to investigate the effect of Ectoine as a storage media on the viability and differentiation potential of human periodontal ligament mesenchymal stem cells ( hPDLMSC s). Materials and Methods hPDLMSC s were obtained from impacted third molar teeth. The cells were isolated, submitted to trilineage differentiation, and characterized by flow cytometer ( FC ). hPDLMSC s were incubated with different media containing Ectoine, complete DMEM (c DMEM ), Ectoine+c DMEM , milk, and tap water for 2, 6, 12, and 24?h. The cells were analyzed by FC for viability, early‐apoptosis, late apoptosis, and necrosis rates. Osteogenic and fibroblastic differentiation in hPDLMSC s were investigated by Alizarin red stain and vimentin expression. Results All treated groups showed significant decreases in cell viability after 2?h. Significant increases were detected in the number of dead cells between 2 and 12?h in all groups except the Ectoine+c DMEM group. The deposition of mineral matrix nodules was significantly higher in cells cultured with Ectoine+c DMEM compared with the other media. Higher vimentin expressions were detected in cells cultured with c DMEM and Ectoine+c DMEM media, respectively. Conclusions Ectoine added to c DMEM media, promoted cell survival plus osteogenic and fibroblastic differentiation of hPDLMSC s.