Cloning, subcellular localization, and expression analysis of VvRING1a from grapevine

摘要

Polycomb group (PcG) proteins within polycomb repressive complex 1 (PRC1) and PRC2 are significant epigenetic regulation factors involved in controlling plant developmental transitions. PRC2 catalyzes H3K27 trimethylation (H3K27me3), and PRC1 recognizes the site of H3K27me3 and catalyzes H2AK119 monoubiquitination to maintain transcription repression. PRC2 components are conserved from plants to animals and have been investigated for a long time. However, the compositions and functions of PRC1 vary between animals and plants. Plant PRC1 has only been confirmed in recent years. Very little research has been conducted on PcG in grapevine (Vitis vinifera 'Cabernet Sauvignon'). Here, we cloned the full-length coding sequence of the VvRING1a gene, which is homologous with the Arabidopsis thaliana PRC1 core component AtRING1a. We then analyzed the structure, subcellular localization, and expression pattern. The tissue-specific expression analysis showed that VvRING1a is expressed in various tissues, including in roots, stems, leaves, flowers, buds, and fruits. This study will be a useful reference for further study of VvRING1a.