Development of a Porcine Model of Coronary Stenosis Using Fully Percutaneous Techniques Suitable For Performing Cardiac Computed Tomography, CT-Perfusion Imaging and Fractional Flow Reserve

摘要

Background The aim of this study was to develop and describe percutaneous coronary angiographic techniques to create a porcine model of acute coronary stenosis with methacrylate plugs that can by assessed using fractional flow reserve (FFR), invasive coronary angiography and coronary computed tomographic (CT) perfusion imaging without introducing artefacts associated with surgical models. Methods Following animal care and institutional approval and using percutaneous coronary catheterisation techni ques within an animal laboratory we introduced precision drilled methacrylate plugs into one of the three main coronary arteries of 10 experimental female pigs. Coronary pressure wire measurements were performed across the experimental stenosis for the calculation of FFR. Invasive coronary angiograms were obtained in stenosed arteries. Animals were transported to a dual source CT scanner (Siemens Healthcare, Forcheim, Germany) and CT perfusion imaging was performed. Results Ten (10) pigs were investigated with seven data sets obtained. Three (3) pigs expired prior to CT imaging secondary to pneumothorax, high grade coronary stenosis with induced cardiac arrhythmia and iatrogenic air embolism. Graded coronary stenosis was produced in six pigs in the LAD (2), LCX (2) and RCA (2) territories and one animal served as a control. Fractional flow reserve ranged from 0.21 to 0.91. Myocardial blood flow derived from dynamic CT perfusion imaging ranged from 3.5 to 136.7 ml/100 ml of tissue/ minute. No artefacts from the deployment of the methacrylate plug, nor the plug itself, were identified. Conclusions Fully percutaneous preparation of a pig model of acute coronary stenosis is feasible and provides subjects for imaging that are free of surgically induced artefact. This technique is substantially less expensive than surgically induced coronary stenosis and can be performed using standard catheterisation techniques with mobile imaging equipment. The technique is extendable to produce multivessel acute coronary stenosis and can be used for multimodality imaging.