Development of a High-speed Super-resolution Confocal Scanner

摘要

Super-resolution microscopy techniques that overcome the spatial resolution limit of conventional light microscopy have been developed and are increasingly used for advanced research in various fields including cell biology. However, their temporal resolution is still low, so it is diff icult to observe the dynamic behaviors of living cells. Furthermore, these existing techniques have restrictions on the dyes that can be used and the specimens that can be observed. Therefore, there is an increasing need for a versatile super-resolution microscopy technique that can visualize the fine structures of living cells with high temporal resolution. To meet this need, Yokogawa has developed a high-speed super-resolution confocal scanner based on its confocal scanner unit (CSU), which is proven to be ideal for observing living cells. This paper outlines the technologies implemented in this new scanner and its applications.