Kinetics of interferon-gamma production and its comparison with anti-listeriolysin O detection in experimental bovine listeriosis.

摘要

The kinetics of interferon gamma (IFN-gamma) production in whole blood culture and its comparison with anti-listeriolysin O (ALLO) detection by ELISA were studied in calves after oral infection with Listeria monocytogenes. Culture filtrate antigen (CFA), listeriolysin O (LLO), and sonicated antigen (SA) were used to prime the peripheral blood mononuclear cells (PBMCs) and the plasma from infected calves. IFN-gamma and ALLO appeared from day 7 after infection. IFN-gamma was detected earlier with LLOthan with SA. The Max50 interleukin (IL-2) activity and IFN-gamma measured in the culture supernatant from PBMCs primed in vitro with different antigens of L. monocytogenes showed high induction of IL-2 and IFN-gamma by CFA, LLO and live antigen. IFN-gamma assay and ALLO detection were used for testing cases of repeat breeding in dairy cattle. It is concluded that detection of IFN-gamma induced by LLO can be used to diagnose listerial infections.