Comparative analysis of dentine and gingival fluid molecular composition and protein conformations during development of dentine caries: A pilot study

摘要

Background: One of the most important yet unresolved tasks facing therapeutic dentistry is the problem of efficient personalised diagnostics of pathological processes in the dentine tissues of human teeth. In this regard, dentine fluid is an ideal screening candidate, which acts as an important factor in the development of dentine caries. However, the serious difficulty and practical impossibility of dentine fluid sampling in vivo is the main obstacle to its use in advancing a new line of diagnostics. Extracting gingival crevicular fluid for diagnosing is a much simpler approach. Therefore, we sought to establish the molecular composition and protein conformation of blood, dentine fluid, and gingival fluid during the development of dentine caries using synchrotron FTIR investigations.