Preparation of liposomes encapsulating beta-carotene using supercritical carbon dioxide with ultrasonication

摘要

beta-Carotene-loaded liposomes were prepared utilizing ultrasonication and supercritical carbon dioxide (SC-0O2) as an alternative to organic solvents. Thermally Z-isomerized)8 -carotene samples containing 2.4, 37, or 87 % Z-isomers were fed with hydrogenated soy phosphatidylcholine. Pressure and temperature were changed in ranges 10-25 MPa and 50-70 C, respectively, and isomerization ratio was changed. Transmission electron microscopy revealed 90-150 nm spherical liposomes. Ultraviolet -visible spectroscopy indicated that)8 -carotene aqueous nanosuspensions formed more effectively when Z-isomer-rich)8-carotene was used as a starting material. Zeta potentials indicated that soy-phosphatidylcholine-based Z -isomer-containing)8 -carotene aqueous nanosuspensions showed good stability. (C) 2020 Elsevier B.V. All rights reserved.