Low-cost mobile open-circuit hood system for measuring gas exchange in small ruminants: from manual to automatic recording

摘要

Improvements of a home-made mobile open-circuit respirometry system for the rapid determination of methane (CH4) and carbon dioxide (CO2) production, oxygen (O-2) consumption and, thereafter, heat production (HP) for small ruminants are described and validated. Upgrades consisted of three main features: utilization of a head hood (replacing the previous face mask); use of a computerized control system, data acquisition and recording for gases and air flux (replacing collecting bags for air sampling); and use of a gas cooler to remove the air sample moisture (replacing the chemical drier (silica gel) approach). Calibration factors were established by injecting nitrogen (N-2) and CO2 in the system into the head hood. Repetitive and consistent values for the calibration factor were obtained for O-2 and CO2 which confirmed the absence of leaks and the good performance of the system. In addition, an experimental test with 12 Manchega female dry sheep was conducted to validate the system. Three diets based on cereal grain, fibrous by-products and alfalfa hay (ALH) were used with four sheep per diet. Metabolizable energy intake was close to metabolizable energy for maintenance. Average HP measured by indirect calorimetry (respiratory quotient (RQ) method) was close to the average HP determined from Carbon-Nitrogen balance (CN method) accounting for 443 and 426 kJ/kg(075) body weight (BW) per day, respectively. Fasting HP was determined by the RQ method with two sheep from the ALH diet accounting for 269 kJ/kg(075) BW per day. The head hood and computerized control, data acquisition and recording as well as the gas cooler improved the system by reducing the labour input without loss of functionality for measuring gas exchange and energy metabolism in small ruminants.