Production and characterization of swine hyperimmune serum against recombinant, common antigens of Gram-negative outer membrane bacteria

摘要

The application of immune serum is one of the most efficient method used formerly in the protection of raised piglets'/weaners' health. The objective of the study was to determine specific antibody response during hyperimmunization of fatteners with a self-prepared subunit vaccine, and to propose production method of immune serum against Gram-negative bacteria antigens. The vaccine was administered every two weeks, 4 times. Individual and pooled serum samples were assayed for IgM, IgG and IgA antibodies against Histophilus somni recombinant Hsp60, H. somni rOMP40 and Pasteurella multocida LPS. Additionally total serum IgG and haptoglobin concentrations were measured.