Cypermethrin resistance conferred by increased target insensitivity and metabolic defoxification in Culex pipiens pallens Coq

摘要

In order to elucidate the molecular mechanisms of cypermethrin resistance in Culex pipiens pollens Coq, the susceptible strain (SS strain) and cypermethrin resistant strain (CR strain) of Ox p. pallens were investigated in this paper. The cypermethrin resistance ratio of CR strain to SS strain was measured by biological assays method, the cDNA sequence of sodium channel was cloned and analyzed. Real-time quantitative RT-PCR was used to detect the expression levels of the detoxification-related genes across between CR strain and SS strain of Cx. p. pallens. Bioassays indicated that CR strain was 283.06 and 80.68-fold resistance to cypermethrin and permethrin as compared to the susceptible strain, respectively. The sequence variability analysis of sodium channel gene between SS strain and CR strain shows that 4 point mutations (R954Q L1023F, S1775G and A1989E) appear on the amino acid sequence of sodium channel of CR strain. The transcriptional levels of CYPEZ10, CYP9M10, CPGSTd1 and CPGSTd2 in the resistant strain are significantly higher than it is in the susceptible. The transcripts of CYP4H34 and E4 esterase have no significant difference between the CR strain and SS strain. The results indicated that sodium channel mutations, combined with elevated levels of P450s and GSTs, are associated with cypermethrin resistance in CR strain. (C) 2017 Elsevier Inc. All rights reserved.