mRNA, rRNA and DNA quantitative stable isotope probing with (H2O)-O-18 indicates use of old rRNA among soil Thaumarchaeota

摘要

RNA is considered to be a short-lived molecule, indicative of cellular metabolic activity, whereas DNA is thought to turn over more slowly because living cells do not always grow and divide. To explore differences in the rates of synthesis of these nucleic acids, we used (H2O)-O-18 quantitative stable isotope probing (qSIP) to measure the incorporation of O-18 into 16S rRNA, the 16S rDNA, amoA mRNA and the amoA gene of soil Thaumarchaeota.