Linking bacterial and eukaryotic microbiota to litter chemistry: Combining next generation sequencing with C-13 CPMAS NMR spectroscopy

摘要

Microbial succession over decomposing litter is controlled by biotic interactions, dispersal limitation, grazing pressure, and substrate chemical changes. Recent evidence suggests that the changes in litter chemistry and microbiome during decomposition are interdependent. However, most previous studies separately addressed the microbial successional dynamics or the molecular changes of decomposing litter. Here, we combined litter chemical characterization by C-13] NMR spectroscopy with next generation sequencing to compare leaf litter chemistry and microbiome dynamics using 30 litter types, either fresh or decomposed for 30 and 180 days.