A simple procedure for the micropropagation of Cistus incanus L. from aseptic seedlings and ex vitro plants

摘要

In the framework of research into the association between C. incanus and its symbiont Tuber melanosporum, a procedure for micropropagating the host plant was required. For seed germination, the use of 10% NaOCl or concentrated sulfuric acid allowedhigh germinability on 0.6% water agar. Transfer to a Woody Plant Medium plus 0.5 mg/litre-1 indolebutyric acid [IBA] for two months resulted in uniform aseptic seedlings. For apical and nodal explants from outdoor plants, a mild disinfection procedure followed by application of antibiotics yielded cultures with bud growth. The best medium for multiplication of apical and nodal segments was MS+0.1 mg litre-1 benzyladenine, while a good medium for rooting was MS unsupplemented with plant growth regulators. To assure acclimatization, rooted plantlets should be allowed to develop canopy and roots for at least 8 weeks prior to potting. A high survival rate was recorded when plants were covered for 2 weeks. Acclimatized plants have been successfully colonized with T. melanosporum.