Effect of branched chain amino acid and leucine supplement on hydrogen peroxide toxicity in albino rabbits

摘要

For two months, groups of 6 albino 4 to 5 month old rabbits each, were given water with 0.02% hydrogen peroxide (H2O2) and standard feed with 0.05, 0.1 or 0.2% branched chain amino acid (BCAA) mix, and 0.1% leucine. Controls were 6 rabbits each givenwater with or without 0.02% H2O2 and standard diet. After fasting 12h, blood was taken at days 30 and 60 for glucose, albumin and total protein values. Compared with normal controls, blood glucose increased ~46-55mg/dl in H2O2 controls. At day 30, therewere also increases in serum glucose in rabbits given BCAA compared with normal controls. However by 60 days in those given 0.1 or 0.2% BCAA, glucose values were comparable with normal values. Compared with normal controls there were very large falls (2.1-2.2mg/dl) in albumin in peroxide controls. There were also decreases in those supplemented BCAA except only in those given 0.1% BCAA by 60 days. Serum TPP declined sharply in peroxide controls (nearly 2 fold) compared with normal controls but in rabbits given 0.1 or 0.2% BCAA we found values similar to normal controls. Findings suggest that BBAA supplements at higher doses may provide some palliative effect on hydrogen peroxide toxicity in rabbits.